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Sutiman Bambang Sumitro
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INDONESIA
Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 8 Documents
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Search results for , issue "Vol 19 No 2 (2014): June 2014" : 8 Documents clear
THE INFLUENCE OF Aloe vera AND XENOGRAFT (XCB) TOWARD OF BONE MORPHO PROTEIN 2 ( BMP2) EXPRESSION AND AMOUNT OF OSTEOBLAST OF ALVEOLAR BONE INDUCED INTO TOOTH EXTRACTION SOCKETS (Cavia cobaya) Utari Kresnoadi; Retno Pudji Rahayu
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 2 (2014): June 2014
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2390.186 KB) | DOI: 10.23869/118

Abstract

Tooth extraction can cause inflammation leading to alveolar ridge resorption. In addition, prominent ridge has crucial role for making denture successfully. Thus, socket preservation is needed to prevent greater alveolar ridge resorption. An innovative material, a combination of Aloe vera and xenograft (XCB), is then considered as a biogenic stimulator that can reduce inflammation, as a result, the growth of alveolar bone is expected to be improved. This research is aimed to prove whether the mixture of Aloe vera and xenograft can stimulate BMP2 and increase osteoblasts. Forty-eight Cavia cobaya animals were divided into eight groups each of which consisted of six animals. The mandibular incisors of those Cavia cobaya animals were then extracted and filled with PEG as Group Control, XCB as Group XCB, Aloe vera as Group Aloe vera, and a combination of Aloe vera +XCB as Group Aloe vera +XCB. Next, the first four groups were sacrificed seven days after extraction, and the second four groups were sacrificed 30 days after extrac-tion. And then, immunohistochemical and histopathology examinations were conducted to examine BMP2 expression and osteoblasts. Based on the re-sult known that the mixture of Aloe vera and xenograft can increase BMP2 expression and amount of osteoblasts. It can be concluded that the mixture of Aloe vera and xenograft can increase BMP2 expression and amount of osteoblast cel . It can be used as an alternative material to increase the growth of alveolar bone after extraction.
PCR APPROACH FOR RAPID DETECTION OF Escherichia coli IN TEMPE USING A SPECIFIC PRIMER Siti Harnina Bintari; Fidia Fibriana; Dewi Mustikaningtyas; Retno Sri Iswari
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 2 (2014): June 2014
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2100.635 KB) | DOI: 10.23869/119

Abstract

Tempe known as a traditional fermented food originated from Indonesia. It has a unique flavour and texture. It also contains high protein and usually serves to substitute meat, fish, or egg as a complement to rice. The manufacture process of Tempe is quite complex and mostly, the traditional process has not employed the hygienic standard. In the process of Tempe making, there are two critical stages of the whole process; i.e. soaking of soybeans and solid state fermentation by Rhizopus sp. During the process, foodborne pathogen bacteria such as Escherichia coli could contaminate the product of Tempe. The bacterial contamination could be revealed through culture dependent methods which is costly, laborious, and time consuming. Therefore, the culture-independent method such as polymerase chain reaction using a specific primer could be applied to detect target microorganism to save time and labour. In this study, thirty-one Tempe samples collected from different manufacturers in Semarang, Central Java, Indonesia were analysed by PCR. In order to obtain the bacterial genomic DNA, a modified Chelex 100-Microwave method was employed. The results of DNA extraction showed that the method was an applicable method. It gave high quantity and quality of DNA; therefore, it could be applied in the PCR reaction. The DNA samples were employed in PCR for detection of Escherichia coli using Ecoli706F/R. It was found that 27 out of 31 samples were detected having Escherichia coli contamination showed by the presence of the amplified product size 706 bp. The application of this method could significantly reduce costs and time of analysis in the laboratory. Further response after E. coli were detected could be employed, including investigation of the critical factors in Tempe manufacturing process which allowed E. coli contamination.
POLYMORPISM ANALYSIS OF IL17RA GENE TO THE IL17RA CONCENTRATION AND CHRONICITY DIFFERENCES IN NEFRITIS LUPUS NL PATIENTS Mahrus Ismail; Hani Susanti; Widodo Widodo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 2 (2014): June 2014
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2323.49 KB) | DOI: 10.23869/120

Abstract

Nefritis lupus (NL) is autoimmune disease and one of serious complication from Systemic Eritematosus Lupus (LES). Clinical manifestation of NL was variated, there are hematuria microscopic asymptomatic until renal failure. In addition of clinical manifestation, the NL disorder degree also measured from histopathological grade. Although, the mechanism that mixed up with pathogenesis of NL was known, however the cytokine also play a role to the disease process. The cytokine that assumpted have a role to the inflammation is IL-17RA, the increase production of IL-17RA influenced to the in-flammation and NL chronicity degree. The aim of this study to analyzed the relationship between polymorphism of IL-17RA gene, blood IL-17RA con-centration with the NL disorder degree. These studies were used cross sectional with control case design. The sample were used 40 patients consist NL pa-tients and Lupus patient without nephritis as a control. The polymorphisms of IL-17RA gene were investigated by using PCR method and gene sequence analysis. The alterations of allele frequency of IL-17RA gene were analyzed by bioinformatics method. The bloods IL-17RA concentration were in-vestigated by ELISA method. The NL chronicity degrees were investigated by NL histopathological grade. The hypothesis were proofed by data normality test and homogeneity test, Chi-square and Odds ratio, Spearman correlation by using SPSS 17.0 for windows. The result of showed that there are sig-nificantly different between IL-17RA gene mutant genotype frequency of NL patient (NL) with the control patient with value OR 8.48. There are sig-nificantly different between G allele of IL-17RA gene of NL patient (case) with the control patient with the value OR 4.17. There are no significantly di-fferent between IL-17RA concentrations of NL patient (case) with the different chronicity value. There are positive correlation with the OR value 4.17 bet-ween IL-17RA concentrations with the chronicity value index.
A STUDY OF TYPE AND INTENSITY OF DISEASE INFECTING BANANA PLANTS Musa sp AT TEGALAGUNG VILLAGE SEMANDING SUBDISTRICT Supiana Dian Nurtjahyani; Devi Shyntya Agustin
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 2 (2014): June 2014
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1159.116 KB) | DOI: 10.23869/121

Abstract

Diseases affecting banana plants are very detrimental to farmers as these can lower production and economic income. The purpose of this study was to determine the type and intensity of the disease affecting banana plants. This research was an observational analytic study that observe and analyze condition or symptoms of diseases affecting banana plants in Tegalagung village, Semanding subdistrict, Tuban as many as 38 samples. Parameters observed were type of disease and measure intensity of the disease, data obtained were analyzed descriptively. Based on the symptoms that occurred on the leaves, the study found four disease types affecting banana plant that were fusarium wilt, bacterial wilt (Blood), Sigatoka leaf spot and stunting disease. The diseases intensity were 50% of Fusarium wilt; 26,66% of bacterial wilt (Blood); 26.32% of Sigatoka leaf spot and 15.38% of stunting disease. Conclusion of the study, the highest intensity of the disease that attacks banana plants is Fusarium wilt as high as 50%.
SLAUGHTER HOUSE SOLID WASTE MANAGEMENT IN INDONESIA Rhenny Ratnawati; Yulinah Trihadiningrum
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 2 (2014): June 2014
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1794.633 KB) | DOI: 10.23869/122

Abstract

he solid slaughter house waste (SSW) in Indonesia is generally disposed of into open dumped landfill. This type of solid waste can cause odor and atmospheric pollution if discharged directly into the environment. Additionally, it may spread disease due to the nesting vectors, and the resulting leachate can lead to groundwater contamination. This paper reviews the characterization of slaughter house (SH) types and SSW generation potential and to review the development of treatment technology of SSW and its application. The SH in Indonesia is divided into 3 classes, namely: 1) SH for large and small ruminants; 2) SH for poultry; 3) SH for pigs. Application technologies in Indonesia include compost and biogas technologies, and the use of rumen content for animal feed. Problem in biogas technology is generally caused by the high nitrogen content in the SSW. The most suitable raw material for biogas production is herbivore waste. The main advantages of using SSW for compost production are: the appropriate characteristics for composting process, free of hazardous contaminant, and appropriate composting technologies are available to reduce environmental problems caused by SSW. In addition, rumen content is considered to be a potential alternative for animal feed because have high content of amino acids (approximately 73.4% of the total protein) and rich in vitamin B complex. Among the disadvantages, the composting process of SSW requires long time period and generate air pollutants, such as ammonia and hydrogen sulphide.
THE EFFECT OF MUNDU FRUIT (Garcinia dulcis (ROXB.) KURZ) METHANOL EXTRACT ON LYPHOPROTEIN PROFILE AND TRYGLISERIDE WHITE RAT (Rattus norvegicus L) Arum Setiawan; Laila Hanum; Elvi Rusmiyanto Pancaning Wardoyo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 2 (2014): June 2014
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (408.291 KB) | DOI: 10.23869/124

Abstract

Garcinia dulcis (Roxb.) Kurz) (Guttiferae) is a tropical fruit tree native to Indonesia and its known as an Asian medicinal plant used in folk medicines. In ethno pharmacology, Garcinia dulcis has been used in traditional medicine, such as anti-inflammatory, anti-tumor, anti-malaria and anti-bacterial. The scientific studies of biological activity of Garcinia dulcis on lipide profile and trigliseride are relatively measly. Therefore, the objectives of this research were to know the effect of methanol extract of Garcinia dulcis fruit on total cholestrol, low density lipoprotein-cholesterol, high density lipoprotein-cholesterol and triglyseride rat hyperlipidemic. These studies using Garcinia dulcis fruit from Banyuasin, South Sumatra. In this observation, 30 male rat from Laboratory and Research Institute for Integrated Testing, Gadjah Mada University, 2 months old were used and divided into five groups randomly. The first group was given high cholesterol and lipid diet, as a normal group. The second group was given high cholesterol, lipid diet and simvastatin 3,6 mg/kg bow/day dosage, as a simvastatin group. The third, fourth and fifth groups was given high cholesterol, lipid diet and methanol extract 1.8; 2.7 and 3.6 mg/kg bow/day dosage. Total cholestrol, low density lipoprotein-cholesterol, high density lipoprotein-cholesterol and triglyseride contains using spectrophotometry method, were observed 1 and 30 days. The differences among groups were analyzed using Anova followed by LSD to locate the differences. LSD analysis showed that Garcinia dulcis methanol extract treatment doses of 3.6 mg/kg bw and simvastatin lowers total cholesterol levels highest in the amount of 32.17 mg/dL and 24.1 mg/dL, significantly different compared with the treatment dose of 1.8 mg/kg bw. Garcinia dulcis fruit methanol extract treatment doses of 3.6 mg/kg bw and simvastatin lowers triglyceride levels by 7.22 mg/dL and 24.58 mg/dL, significantly different compared with the treatment dose of 1.8 mg/kg bw and controls. Garcinia dulcis fruit extract dose of 3.6 mg/dL lower low density lipoprotein-cholesterol highest of 16.45 mg/dL. Garcinia dulcis fruit methanol extract dose of 3.6 mg/kg bw was also able to increase the levels of high density lipoprotein-cholesterol by 5.62 mg/dL. The results showed that the fruit of Garcinia dulcis contain compounds that have potential as agents antihiperlipidemia and presumably through inhibition of the enzyme HMG-CoA reductase.
BIOPHYSICS ENVIRONMENTAL CONDITIONS OF SWAMP BUFFALO Bubalus bubalis PAMPANGAN IN DISTRICT RAMBUTAN SOUTH SUMATERA Yuanita Windusari; Erwin Nofyan; Mustafa Kamal; Laila Hanum; Rahmat Pratama
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 2 (2014): June 2014
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (654.157 KB) | DOI: 10.23869/125

Abstract

Swamp buffalo (Bubalus bubalis) is a germ plasm specific of Pampangan and endemic in South Sumatera with low productivity and limited distribution. The aims of this study was to obtain information regarding biophysical conditions in the central areas of swamp buffalo in South Sumatera. The method used is purposive sampling method. Data collected in the form of quantitative and qualitative. Primary data were obtained through direct observation, interviews breeders selected as respondents while secondary data obtained from various related. The data obtained are presented descriptively and data tabulation. Productivity of swamp buffalo Pampangan can be increased by managing and maintaining habitat conditions although traditional maintenance. The results of observations of the biophysical condition of swamp buffalo (B. bubalis) Pampangan showed that habitat of swamp buffalo Pampangan consists of dominated by lowland swamp area is overgrown with shrubs and grass. The conclution of the research are productivity and population of swamp buffalo (B. bubalis) pampangan as specific plasma nutfah of South Sumatra can be improved by studying the characteristics and preferred habitat of the buffalo, although developed in a traditional farms but is good enough and so need to be developed, grass is most preferred by swamp buffalo Pampangan derived from ‘Kumpai’ grass group, and ‘Kasur’grass and ‘Kumpai’ grass is the dominant grass type found in habitat swamp buffalo Pampangan.
EFFECT OF GROWTH CONDITIONS ON BIOFILM FORMATION BY PHENOL-DEGRADING BACTERIA ISOLATED FROM POLLUTED AND NON-POLLUTED SOURCES Arifah Khusnuryani; Erni Martani; Tri Wibawa; Jaka Widada
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 2 (2014): June 2014
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (503.217 KB) | DOI: 10.23869/126

Abstract

Our previous research have isolated four phenol degrading bacteria. There are ATA6, DOK135, and DL120 which isolated from polluted source (hospital wastewater), also HP3 which isolated from non polluted source (peat soil). The purpose of this research is to analyze the effect of some environmental factors on the ability of four isolates to form biofilm. The environment factors were varied, such as growth medium, incubation temperature, and medium pH. Biofilm formation was measured using microtiter plate and crystal violet method, and the absorbance was read with microtiter auto reader at wavelenght 490 nm. The result showed that ATA6 was a strong biofilm former, DOK135 and HP3 were moderate biofilm former, and DL120 was a weak biofilm former. The results indicate that there is variation in the ability of selected isolates to form biofilm on various environmental factors. Generally, the isolates formed thicker biofilm in TSB medium which is a complex medium that provide more complete nutrient and formed biofilm optimally at 30oC. ATA6 formed biofilm optimally at pH 7 and HP3 at pH 9, while pH treatment did not affect on isolates DOK135 and DL120 to form biofilm.

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